基因数据处理4之BWA索引内存不够

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基因数据处理4之BWA索引内存不够


hadoop@Mcnode1:~/cloud/adam/xubo/data/data_HDFS/Ref/RefFromBwaGit$ bwa index GRCH38.fa 
[bwa_index] Pack FASTA... 37.70 sec
[bwa_index] Construct BWT for the packed sequence...
[BWTIncCreate] textLength=6418915856, availableWord=463658232
[BWTIncConstructFromPacked] 10 iterations done. 100000000 characters processed.
[BWTIncConstructFromPacked] 20 iterations done. 200000000 characters processed.
[BWTIncConstructFromPacked] 30 iterations done. 300000000 characters processed.
[BWTIncConstructFromPacked] 40 iterations done. 400000000 characters processed.
[BWTIncConstructFromPacked] 50 iterations done. 500000000 characters processed.
[BWTIncConstructFromPacked] 60 iterations done. 600000000 characters processed.
[BWTIncConstructFromPacked] 70 iterations done. 700000000 characters processed.
[BWTIncConstructFromPacked] 80 iterations done. 800000000 characters processed.
[BWTIncConstructFromPacked] 90 iterations done. 900000000 characters processed.
[BWTIncConstructFromPacked] 100 iterations done. 1000000000 characters processed.
[BWTIncConstructFromPacked] 110 iterations done. 1100000000 characters processed.
[BWTIncConstructFromPacked] 120 iterations done. 1200000000 characters processed.
[BWTIncConstructFromPacked] 130 iterations done. 1300000000 characters processed.
[BWTIncConstructFromPacked] 140 iterations done. 1400000000 characters processed.
[BWTIncConstructFromPacked] 150 iterations done. 1500000000 characters processed.
[BWTIncConstructFromPacked] 160 iterations done. 1600000000 characters processed.
[BWTIncConstructFromPacked] 170 iterations done. 1700000000 characters processed.
[BWTIncConstructFromPacked] 180 iterations done. 1800000000 characters processed.
[BWTIncConstructFromPacked] 190 iterations done. 1900000000 characters processed.
[BWTIncConstructFromPacked] 200 iterations done. 2000000000 characters processed.
[BWTIncConstructFromPacked] 210 iterations done. 2100000000 characters processed.
[BWTIncConstructFromPacked] 220 iterations done. 2200000000 characters processed.
[BWTIncConstructFromPacked] 230 iterations done. 2300000000 characters processed.
[BWTIncConstructFromPacked] 240 iterations done. 2400000000 characters processed.
[BWTIncConstructFromPacked] 250 iterations done. 2500000000 characters processed.
[BWTIncConstructFromPacked] 260 iterations done. 2600000000 characters processed.
[BWTIncConstructFromPacked] 270 iterations done. 2700000000 characters processed.
[BWTIncConstructFromPacked] 280 iterations done. 2800000000 characters processed.
[BWTIncConstructFromPacked] 290 iterations done. 2900000000 characters processed.
[BWTIncConstructFromPacked] 300 iterations done. 3000000000 characters processed.
[BWTIncConstructFromPacked] 310 iterations done. 3100000000 characters processed.
[BWTIncConstructFromPacked] 320 iterations done. 3200000000 characters processed.
[BWTIncConstructFromPacked] 330 iterations done. 3300000000 characters processed.
[BWTIncConstructFromPacked] 340 iterations done. 3400000000 characters processed.
[BWTIncConstructFromPacked] 350 iterations done. 3500000000 characters processed.
[BWTIncConstructFromPacked] 360 iterations done. 3600000000 characters processed.
[BWTIncConstructFromPacked] 370 iterations done. 3700000000 characters processed.
[BWTIncConstructFromPacked] 380 iterations done. 3800000000 characters processed.
[BWTIncConstructFromPacked] 390 iterations done. 3900000000 characters processed.
[BWTIncConstructFromPacked] 400 iterations done. 4000000000 characters processed.
[BWTIncConstructFromPacked] 410 iterations done. 4100000000 characters processed.
[BWTIncConstructFromPacked] 420 iterations done. 4200000000 characters processed.
[BWTIncConstructFromPacked] 430 iterations done. 4300000000 characters processed.
[BWTIncConstructFromPacked] 440 iterations done. 4400000000 characters processed.
[BWTIncConstructFromPacked] 450 iterations done. 4500000000 characters processed.
[BWTIncConstructFromPacked] 460 iterations done. 4600000000 characters processed.
[BWTIncConstructFromPacked] 470 iterations done. 4700000000 characters processed.
[BWTIncConstructFromPacked] 480 iterations done. 4800000000 characters processed.
[BWTIncConstructFromPacked] 490 iterations done. 4900000000 characters processed.
[BWTIncConstructFromPacked] 500 iterations done. 5000000000 characters processed.
[BWTIncConstructFromPacked] 510 iterations done. 5100000000 characters processed.
[BWTIncConstructFromPacked] 520 iterations done. 5200000000 characters processed.
[BWTIncConstructFromPacked] 530 iterations done. 5300000000 characters processed.
[BWTIncConstructFromPacked] 540 iterations done. 5400000000 characters processed.
[BWTIncConstructFromPacked] 550 iterations done. 5500000000 characters processed.
[BWTIncConstructFromPacked] 560 iterations done. 5600000000 characters processed.
[BWTIncConstructFromPacked] 570 iterations done. 5700000000 characters processed.
[BWTIncConstructFromPacked] 580 iterations done. 5798188880 characters processed.
[BWTIncConstructFromPacked] 590 iterations done. 5886472096 characters processed.
[BWTIncConstructFromPacked] 600 iterations done. 5964934432 characters processed.
[BWTIncConstructFromPacked] 610 iterations done. 6034667936 characters processed.
[BWTIncConstructFromPacked] 620 iterations done. 6096643264 characters processed.
[BWTIncConstructFromPacked] 630 iterations done. 6151723072 characters processed.
[BWTIncConstructFromPacked] 640 iterations done. 6200674128 characters processed.
[BWTIncConstructFromPacked] 650 iterations done. 6244177920 characters processed.
[BWTIncConstructFromPacked] 660 iterations done. 6282840176 characters processed.
[BWTIncConstructFromPacked] 670 iterations done. 6317199264 characters processed.
[BWTIncConstructFromPacked] 680 iterations done. 6347733664 characters processed.
[BWTIncConstructFromPacked] 690 iterations done. 6374868704 characters processed.
[BWTIncConstructFromPacked] 700 iterations done. 6398982368 characters processed.
[BWTIncConstructFromPacked] 710 iterations done. 6418915856 characters processed.
[bwt_gen] Finished constructing BWT in 710 iterations.
[bwa_index] 3308.16 seconds elapse.
[bwa_index] Update BWT... [bwt_bwtupdate_core] Failed to allocate 3209457988 bytes at bwtindex.c line 135: Cannot allocate memory
hadoop@Mcnode1:~/cloud/adam/xubo/data/data_HDFS/Ref/RefFromBwaGit$ ls
FromUrl.txt  GCA_000001405.15_GRCh38_full_analysis_set.fna  GRCH38.fa  GRCH38.fa.amb  GRCH38.fa.ann  GRCH38.fa.bwt  GRCH38.fa.pac  hs37d5.fa.gz

不知道可不可以划分建索引?




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